Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy

Seethapathy G.S, Tadesse M, Santhosh Kumar J. U, Srikanth G, Vasudeva R, Malterud K. M , Uma Shaanker R, Boer H. J, Ravikanth G and Wangensteen H. 2018. Authentication of Garcinia fruits and food supplements using DNA barcoding and NMR spectroscopy. Scientific Reports
Gopalakrishnan Saroja Seethapathy, Margey Tadesse, Santhosh Kumar J. Urumarudappa, Srikanth V. Gunaga, Ramesh Vasudeva, Karl Egil Malterud, Ramanan Uma Shaanker, Hugo J. de Boer, Gudasalamani Ravikanth & Helle Wangensteen
Other Details: 
Publication name: 

Garcinia L. (Clusiaceae) fruits are a rich source of (−)-hydroxycitric acid, and this has gained considerable attention as an anti-obesity agent and a popular weight loss food supplement. In this study, we assessed adulteration of morphologically similar samples of Garciniausing DNA barcoding, and used NMR to quantify the content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in raw herbal drugs and Garcinia food supplements. DNA barcoding revealed that mostly Ggummi-gutta (previously known as Gcambogia) and Gindicawere traded in Indian herbal markets, and there was no adulteration. The content of (−)-hydroxycitric acid and (−)-hydroxycitric acid lactone in the two species varied from 1.7% to 16.3%, and 3.5% to 20.7% respectively. Analysis of ten Garcinia food supplements revealed a large variation in the content of (−)-hydroxycitric acid, from 29 mg (4.6%) to 289 mg (50.6%) content per capsule or tablet. Only one product contained quantifiable amounts of (−)-hydroxycitric acid lactone. Furthermore the study demonstrates that DNA barcoding and NMR could be effectively used as a regulatory tool to authenticate Garciniafruit rinds and food supplements.

Full Text URL:
Year of publication: 
Dr. G Ravikanth
Dr R Uma Shaanker
Research and Development of an herbal and fish product authentication service using DNA barcoding. Funded by Department of Biotechnology (DBT) New Delhi
PDF Upload: 
Non-ATREE Publication(Y/N):